Abstract Details

Title Assessing Post-Stroke Cardiac Dysfunction in the Insular Ischemic Stroke Rat Model

Topic Cerebrovascular Disease and Interventional Neurology

Presentation(s) S22 - Stroke Genetics, Cellular Responses, and Animal Models (4:58 PM-5:09 PM)

Poster/Presentation
Number 009

Background Patients who have suffered an ischemic stroke with damage to the right insular cortex (IC) often develop post-stroke cardiac complications. However, the pathophysiology and time course of these post-stroke myocardial changes require further investigation which is complicated by a lack of pre-clinical models.

Objective To assess the time course of post-stroke cardiac fibrosis and inflammation in a rat model of selective insular ischemic stroke.

Design/Methods IC ischemic stroke was induced in six-month-old Wistar rats via unilateral stereotaxic injection of endothelin-1 into the right IC (n=6/surgical group). Control rats received a phosphate-buffered saline (PBS) injection into the right IC (n=6/surgical group). To establish a time course of cardiac dysfunction, hearts were histologically examined at 6, 24 hours, 7, 14 and 28 days post-stroke for fibrosis (Masson’s Trichrome stain), inflammation (CD45+, myeloperoxidase, CD3+, CD45R, CD68+ immunostaining) and endothelial dysfunction (phosphorylated endothelial nitric oxide synthase (eNOS)). Areas of interest included the 4 heart chambers and pulmonary vein/left atrium border (PV-LA border).

Results Results from this study showed that focal IC ischemic stroke led to neutrophil infiltration at 6 hours, followed by T lymphocyte infiltration at 7 days at the PV-LA border. Left atrial tissue experienced long-term fibrosis at 28 days following focal IC stroke. Further, B lymphocyte infiltration was seen at 28 days post-stroke in left ventricular tissue.

Conclusions These findings provide insight into the progression of post-stroke cardiac changes and suggest that inflammation is a treatable target to prevent post-stroke myocardial injury.

Authors/Disclosures
Luciano A. Sposato, MD (London Health Sciences Centre) PRESENTER	Dr. Sposato has received personal compensation in the range of $5,000-$9,999 for serving on a Speakers Bureau for Pfizer. Dr. Sposato has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for Bayer. Dr. Sposato has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for Boehringer Ingelheim. Dr. Sposato has received personal compensation in the range of $500-$4,999 for serving on a Speakers Bureau for Gore.
	No disclosure on file
Stella Iankov, MD	No disclosure on file
	No disclosure on file
	No disclosure on file
Shawn Whitehead	No disclosure on file

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